Abstract:

A method was established to determine sulfonamides in eel muscle by post-column derivatization high performance liquid chromatography. The sulfonamides residues in eel were extracted with acetonitrile and water, isolated by liquid-liquid partition and solid-phase extraction, then determined by post-column derivatization high performance liquid chromatography. The sulfonamides were separated by C18 column, converted to fluorescent derivatives by post-column derivatization with fluorescamine, detected by fluorescence detector. The limits of quantification is 2.0 μg/kg. The correlation coefficients of linear calibration curves are over 0.9998 within the concentration range of 0.005 to 0.2 μg/ml. For the 2.0 to 50.0 μg/kg spiked eel sample, both within-day and day-to-day recoveries are from 85.1% to 90.2%, and RSDs are from 2.74% to 6.04%. The experimental results presented above indicate that this method can be used to detect the low concentration sulfomamides in eel muscle.

Key words: sulfonamides, post-column derivatization, eel